Intact parasite-antigen ELISA test: a new dimension for serodiagnosis of Amoebiasis

Multiple-antigen ELISA for melioidosis - a novel approach to the improved serodiagnosis of melioidosis

BACKGROUND Burkholderia pseudomallei, the causative agent of melioidosis, is endemic to Southeast Asia and northern Australia. Clinical manifestations of disease are diverse, ranging from chronic infection to acute septicaemia. The current gold standard of diagnosis involves bacterial culture and identification which is time consuming and often too late for early medical intervention. Hence, ra...

An immobilization test for amoebiasis.

The demonstration of antibodies in syphilitic sera that will immobilize Treponema pallidum (Nelson and Mayer, 1949) led Cole and Kent (1953) to investigate and demonstrate that immune rabbit sera were capable of immobilizing Entamoeba histolytica, and to suggest that sera from human cases of amoebiasis might show the same phenomenon. Their rabbit sera did not show immobilizing activity to a hig...

Development of an ELISA Test for Detection of Anti FMD Virus Type A87/IRN Antibody Using Irradiated Antigen

Background and Aims: The aim of this study was application of gamma irradiated FMDV antigen for liquid phase blocking sandwich ELISA (B-ELISA) to quantify guinea pig anti FMDV type A87/IRN antibody. Methods: FMDV type A87/IRN was propagated to a titer of 107.5/ml TCID50 and then irradiated by gamma irradiator. The irradiated 146 S antigen was purified by sucrose gradient centrifugation after in...

IgG ELISA for invasive amoebiasis in endemic areas

An IgG ELISA was evaluated as a serological test for the diagnosis of invasive amoebic disease in an endemic area. Sixteen patients with proven amoebic dysentery, 22 with suspected amoebic dysentery and 21 with amoebic liver abscesses were studied. Control subjects were 26 asymptomatic amoebic cyst passers, 13 patients with giardiasis, 26with enteric fever and 31 individuals with no evidence of...

Improvement of Leishmania major Amastigote ELISA Test Using Various Antigen Antibody Dissociating Agents and Pepsin Digestion.